PPARγ Agonists Down-Regulate the Expression of mRNA during Adipogenesis

Amanda L. Peretich1, Maria Cekanova2, Sarah Hurst1, Seung J. Baek2, Madhu S. Dhar*, 1
1 Departments of Large Animal Clinical Sciences University of Tennessee, Knoxville, TN 37996, USA
2 Pathology, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996, USA

© 2009 Peretich et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the College of Veterinary Medicine, 2407 River Drive, Knoxville, TN 37996, USA; Tel: +1 865 974 5691; Fax: +1 865 974 5773; E-mail:


We have shown that Atp10c, a type 4 P-type ATPase, is a strong candidate affecting glucose and lipid metabolism in humans and mice. Atp10c is a putative phospholipid translocase associated with cell signaling and intracellular protein trafficking. In order to examine the biological role of Atp10c, semiquantitative reverse transcriptase – polymerase chain reaction was carried out. Atp10c mRNA is expressed in 3T3-L1 cells and in primary preadipocytes and adipocytes generated from mice. mRNA is regulated during fat cell differentiation and modulated by PPARγ agonists and antagonists as well as by hormonal factors (insulin and dexamethasone). Atp10c expression is regulated by both the process of adipocyte differentiation and by effectors of fat and glucose metabolism. Taken together these data along with the published phenotype of the Atp10c heterozygote mice suggest that ATP10c is a newly identified protein with a possible biological role in the development of obesity and obesity-related metabolic disorders.